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High-Throughput Lentiviral Library Construction

High-Throughput Lentiviral Library Construction

Automation-Assisted Workflow for High-Throughput Lentiviral Library Construction

In-silico tools enabling CRISPR guide selection against hundreds of genes, coupled with an integrated high-throughput automation platform for lentivirus manufacture offers the potential for the rapid generation of large arrayed lentiviral libraries. At OXGENE, we have employed a bioinformatics-driven approach to CRISPR guide selection and have rigorously optimised processes for lentiviral manufacture in 96-well plate format to achieve maximal viral yields. OXGENE’s integrated automated platform (based on Hamilton and Hudson Robotics technologies) brings together automated plasmid construction, DNA preparation, transfections, and viral titration, enabling the rapid, reliable, and consistent generation of large arrayed lentiviral libraries.

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Connex revolutionises DNA design, optimization and assembly by ensuring the highest levels of quality control, efficiency and project management available for any client.

OXGENE has fully integrated all its smart labs and robotic production processes within a single technology platform that allows real-time project updates for our customers.