Skip to content

XLenti™ packaging and producer cell lines

XLenti™ stable clonal packaging and producer cell lines offer easy scale up, reduced cost of goods and reduced process complexity.

Plasmid Engineering

XLenti™ packaging and producer cell lines contain the same proven genetic system as the XLenti™ transient system, facilitating a smooth transition to this more scalable technology, and retaining all the optimisation benefits developed for the transient system.

Process Development

Our up- and downstream process development experts improve titre through process optimisation, then support you to expedite on-boarding of technologies and facilitate technology transfer.

Evaluate and License

XLenti™ stable lentiviral packaging and producer cell lines provide scalable and efficient systems for high volume, high titre lentiviral production. XLenti™ packaging and producer cell lines are available for evaluation and license now.

Talk to our team about scalable lentiviral production

Get in touch
XLenti™ packaging  cell lines

OXGENE has successfully developed a suspension-mode, stable lentiviral packaging cell line platform using a proprietary  Tet-inducible system. This demonstrates high titre lentiviral production with single plasmid transfection in both shake  flask and bioreactor.

Our packaging cell lines are GMP-compliant and provide a  simple, flexible platform from which to test multiple transgenes, or variants of a transgene, for  further development.

We use a fully defined, serum/animal component free process to generate both our packaging and producer GMP-compliant HEK293 cell lines, which do  not contain the SV40 Large-T antigen and remain stable in the absence  of  antibiotic selection, minimising the regulatory risks in transitioning to clinical use. 

View our most recent poster here, or check out our recent GEN webinar on demand.

XLenti™ producer  cell lines

Our packaging cell lines also form the basis of our GMP-compliant,  animal component free producer cell lines, which  permit transfection-free, large-scale production of lentiviral vectors for cell or     gene therapies.  This  reduces the overall  cost of goods and process complexity involved in clinical-scale viral vector production, while  improving scalability and system  reproducibility. 

A further advantage of   producer cell lines is the added potential for process development   to improve viral titres once we've stably integrated your  gene of interest , free from the process limitations imposed by transfection. Overall, lentiviral producer cell lines allow  the    consistent production of large quantities of lentiviral vector at lower cost.

OXGENE’s producer cell lines use our proprietary XLenti™ genetic system, which has codon optimised promoter and packaging sequences for increased translation efficiency, Tet inducible VSV-G and Gag-Pol expression, and low homology between expression cassettes for an improved safety profile.

We use a fully defined, serum/animal component free process to generate both our packaging and producer GMP-compliant HEK293 cell lines. Cell lines do  not contain the SV40 Large-T antigen and remain stable in the absence  of  antibiotic selection, minimising the regulatory risks in transitioning to clinical use. 

View our most recent poster here, or check out our recent GEN webinar on demand.

From our experts to yours: our scientists offer ongoing and detailed technical support so you can have complete confidence in the performance of our technologies once transferred to your own facilities.

Get in touch about evaluating or licensing our XLenti™ packaging and producer cell lines.

Send us a message

We use cookies to give you the best experience of using this website. By continuing to use this site, you accept our use of cookies. Please read our Cookie Policy for more information.

Our single technology platform that fully connects our smart labs with our customer service proposition.

Login

Connex revolutionises DNA design, optimization and assembly by ensuring the highest levels of quality control, efficiency and project management available for any client.

OXGENE has fully integrated all its smart labs and robotic production processes within a single technology platform that allows real-time project updates for our customers.