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Adenovirus

Optimised adenovirus engineering, production cell lines and proprietary TERA system.

Design and optimise

We design, assemble and produce adenoviral vectors tailored to your application.

Scale up

Our process development experts support you to expedite on-boarding of technologies and to facilitate technology transfer to your laboratories or to a partner CMO.

License for manufacture

We provide a HEK293 and HEK293TetR suspension cell line to facilitate industry-leading performance in adenoviral manufacture.

OXGENE can design, assemble and produce adenoviral vectors of any genetic composition to facilitate both gene therapy and oncolytic applications.

Adenovirus at the edge of impossible

OXGENE has developed a bespoke, GMP-compliant, suspension HEK293TetR cell line for amplification of cytotoxic adenoviral vectors. This animal component and antibiotic free system shows improved viral titre when benchmarked against industry-standard alternatives and allows optimisation of adenoviral production even for transgenes that reduce cell viability. Our vector systems are also available for licensing, so gene therapy developers can proceed without freedom-to-operate or traceability/regulatory concerns, and simultaneously reduce their therapeutic cost of goods.

TERA System

OXGENE has engineered the adenoviral genome to allow temporal control and separation of the adenoviral life cycle. This enables adenoviral helper function during AAV manufacture while completely removing adenoviral particle contamination.

We have built a switchable negative feedback loop into the viral genome to prevent structural gene expression. This system, called ‘Tetracycline-Enabled Repressible Adenovirus’ (TERA), allows normal adenovirus replication in the presence of doxycycline, but represses viral particle production in its absence.

The system has been validated through transient AAV5 production, and is available for early-access evaluation, as well being further developed to complement our in-house stable cell line solutions.

OXGENE is also conducting early-access and collaborative projects to explore further applications of the TERA system. Please email business@oxgene.com to find out more.

HEK293TetR Cell Line

OXGENE has successfully developed a GMP-compliant suspension HEK293TetR cell line for amplification of cytotoxic adenoviral vectors. 

We have demonstrated the functionality of this cell line through our partnership with Vaccitech, and through partner CMOs. In all cases this outperformed commercial alternatives and demonstrates improved scalability and full stability in the absence of antibiotics.


 

AAV Engineering

Optimised AAV Design, Precision Engineering and Scalable Production

Lentivirus Engineering

Increased Scalability and Titer using our Lentiviral Packaging Cell Line

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Connex revolutionises DNA design, optimization and assembly by ensuring the highest levels of qualify control, efficiency and project management available for any client.

OXGENE has fully integrated all its smart labs, machine learning and robotic production processes within a single technology platform that allows real-time project updates for our customers.