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Transient AAV production

Industry leading technology platform for flexible, small-scale gene therapy production

Plasmid and Cell Line Engineering

Our proprietary reconfigured Rep-Cap and modified Ad5 helper plasmids plus clonal suspension cell line offers improved AAV yield.

Process Development

We help optimise processes for pre-clinical manufacture of gene therapy vectors in our ISO:9001 accredited lab. We manufacture up to 10L scale, before assisting technology transfer to you own facilities or CMO.

Evaluate and License

Our industry leading transient AAV production platform, including optimised plasmids and GMP banked suspension cell line are available to evaluate and license.

OXGENE's AAV system is superior to off-the-shelf plasmid systems for AAV titres

Andy Topping, Chief Scientific Officer at FUJIFILM Diosynth Biotechnologies

Talk to the team about high titre AAV production.

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Transient AAV production

Our transient expression platform comprises optimised proprietary AAV plasmids and our GMP-banked clonal suspension HEK293 cell line. We have reconfigured the Rep and Cap genes to place Cap under the control of a strong CMV promoter, while Rep is expressed  at a lower level via an EMCV internal ribosome entry site. We've also boosted expression of Assembly Activating Protein (AAP) from within the capsid ORF. This results in a higher viral titre, better per cell rAAV productivity and improved full:empty vector ratio.   We've also improved  adenoviral helper functionality by simplifying    the Ad5 helper plasmid. 

View our most recent  rAAV vector system, and AAV production posters.

From our experts to yours: our scientists offer ongoing and detailed technical support so you can have complete confidence in the performance of our technologies once transferred to your own facilities

Find out more about evaluating our industry leading transient AAV production platform.

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Our single technology platform that fully connects our smart labs with our customer service proposition.

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Connex revolutionises DNA design, optimization and assembly by ensuring the highest levels of quality control, efficiency and project management available for any client.

OXGENE has fully integrated all its smart labs and robotic production processes within a single technology platform that allows real-time project updates for our customers.