CRISPR Libraries & Screening
OXGENE's expertise in genetic engineering advances discovery for cell and gene therapy pioneers.
Libraries and Screening
CRISPR screens - performed against either the whole genome or specific gene families - allow unbiased discovery of genes that are either essential for cell survival or that confer a particular phenotype, such as drug resistance or sensitivity. This makes them an ideal tool for the identification of target antigens; CRISPR screens offer infinitely more efficient and reliable target identification than can be found by other means such as searches through the literature or genomics datasets.
OXGENE's CRISPR screening solutions can powerfully advance your clinical therapy or drug development programs. We enable a broad project scope that covers custom building your screen from library design and cell line selection through to bioinformatic analysis and validation.
We provide both "off the shelf" and bespoke plasmid and lentiviral libraries, tailored to your screening requirements. We use proprietary CRISPR guide design algorithms for knockout (KO), activation (CRISPRa) and repression (CRISPRi) to ensure high fidelity library construction. We have extensive experience in generating best in class pooled gRNA libraries ranging from 2,500 - 113,000 guides, and arrayed libraries in plate format (plasmid and lentivirus) in excess of 1,000 guides. We apply our extensive automation and informatics know-how to facilitate CRISPR library generation.
Our "off the shelf" pooled libraries include both KO and CRISPRa libraries targeting the whole genome. We offer custom libraries targeting specific gene families and pathways including the kinome, apoptosis, cell cycle, ubiquitin enzymes and druggable genome.
View our most recent CRISPR screening poster.
We put a strong emphasis on communication with our partners, who remain in direct contact with both the project manager and scientific team throughout the project.