We integrate bioinformatics and high-throughput gene editing technology to create a streamlined workflow for automated cell line production.
CRISPR at the edge of impossible
OXGENE provides a complete high-throughput CRISPR engineering platform to meet the ever-increasing demand for custom cell line manufacture, new disease models and novel therapeutics.
From sgRNA library design using proprietary algorithms to automated single cell deposition, growth monitoring and NGS analysis, our high-throughput, automated, scalable robotic genome engineering solution for both gene knock-out and complex modifications offers an unparalleled level of quality and capacity for our partners.
Libraries and Screening
OXGENE has developed proprietary CRISPR design algorithms for knock-out, knock-in, activation and repression to maximise on-target CRISPR activity. We couple this design to our high fidelity library construction service and provide unique dual-format plasmid and lentiviral libraries.
We combine our excellence in vector construction with state-of-the-art bioinformatics (including in-house gRNA design tools, automated primer design and best-in-class algorithms) to optimize the performance of large-scale high-quality pooled gRNA libraries ranging from 2,500-100,000 guides, and arrayed libraries in plate format in excess of 1,000 guides.
In addition to our whole genome library, we offer libraries targeting specific gene families and pathways including the kinome, apoptosis, cell cycle, ubiquitin enzymes and the druggable genome.
Our automated cell culture and phenotypic analysis systems facilitate our cutting-edge CRISPR library screening solutions, and we can apply them in formats such as CRISPR knock-out, CRISPRa and CRISPRi.
Our CRISPR screening platform identifies genetic drivers of drug resistance or sensitivity in different diseases and powerfully advances clinical therapy and drug development programs. We perform this time-consuming work from design to screen analysis giving customers the time to focus on crucial research questions.
CRISPR High-throughput/ KO/Complex Modifications
We provide the world’s first high-throughput, automated, scalable robotic genome engineering platform for CRISPR gene knock-out and complex modifications.
We have optimised and quality-assured each step of our proprietary CRISPR-Cas9 workflow for high-throughput and large-scale demands. However, it is a flexible platform that is easily adapted to specialised, small-scale projects.
Our high production capacity for modified cell lines means we can offer our partners an unparalleled number of gene edited cell lines.
This is only the beginning: our expanding capacity and development in non-standard cell lines (e.g. iPSC) with complex modifications will provide further opportunities for automation and growth.
We are very pleased to join forces with a company of the calibre of Oxford Genetics. They bring excellent expertise in cell biology, gene editing and workflow automation.