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XLenti™ packaging and producer cell lines

Industry leading technologies and services

Our XLenti™ stable clonal packaging and producer cell lines offer easy scale up, reduced cost of goods and reduced process complexity. XLenti™ packaging and producer cell lines contain the same proven genetic system as the XLenti™ transient system, facilitating a smooth transition to this more scalable technology, and retaining all the optimisation benefits developed for the transient system.

We offer expert cloning and engineering services to customise and optimise plasmids for expression of your gene of interest. Our process development services optimise for pre-clinical lentiviral manufacture in our ISO:9001 accredited lab, and we manufacture up to 10L scale, before assisting technology transfer to your own facilities or CMO.

"OXGENE's XLenti™ packaging and producer cell lines offer a cost-effective solution for large scale cell therapy manufacture"

Ryan Cawood, Chief Executive Officer, OXGENE

XLenti™ packaging  cell lines

OXGENE has successfully developed a suspension-mode, stable lentiviral packaging cell line platform using a proprietary Tet-inducible system. This demonstrates high titre lentiviral production with single plasmid transfection in both shake flask and bioreactor.

Our packaging cell lines, containing Inducible VSV-G, Gag-Pol and Rev, have been developed with full traceability from a GMP banked cell line, and provide a simple, flexible platform from which to test multiple transgenes, or variants of a transgene, for further development.

We use a fully defined, serum/animal component free process to generate both our packaging and producer GMP-compliant HEK293 cell lines, which do not contain the SV40 Large-T antigen and remain stable in the absence of antibiotic selection, minimising the regulatory risks in transitioning to clinical use.

View our most recent poster here.

XLenti™ producer cell lines

Our packaging cell lines also form the basis of our animal component free custom producer cell lines, derived from a GMP banked master cell line using our automated platform to screen thousands of single cell clones. These permit transfection-free, large-scale production of lentiviral vectors for cell or gene therapies. This reduces the overall cost of goods and process complexity involved in clinical-scale viral vector production, while improving scalability and system reproducibility.

A further advantage of producer cell lines is the added potential for process development to improve viral titres once we've stably integrated your gene of interest, free from the process limitations imposed by transfection. Overall, lentiviral producer cell lines allow the consistent production of large quantities of lentiviral vector at lower cost.

OXGENE’s producer cell lines use our proprietary XLenti™ genetic system, which is optimised for translation efficiency, has Tet inducible VSV-G and Gag-Pol expression, and low homology between expression cassettes for an improved safety profile.

We use a fully defined, serum/animal component free process to generate both our packaging and producer GMP-compliant HEK293 cell lines. Cell lines do not contain the SV40 Large-T antigen and remain stable in the absence of antibiotic selection, minimising the regulatory risks in transitioning to clinical use.

View our most recent poster here.

From our experts to yours: we offer comprehensive technical support, so you can have complete confidence in our technologies.

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WuXi Advanced Therapies is a global Contract Testing, Development and Manufacturing Organization (CTDMO) dedicated to accelerating progress and time to market for cell and gene therapies.

INTEGRATED GMP MANUFACTURING AND TESTING

XLenti™ Transient Production

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For flexible lentiviral production

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