XLenti™ packaging and producer cell lines
"OXGENE's XLenti™ packaging and producer cell lines offer a cost-effective solution for large scale cell therapy manufacture"
Ryan Cawood, Chief Executive Officer, OXGENE
XLenti™ producer cell lines
Our packaging cell lines also form the basis of our animal component free custom producer cell lines, derived from a GMP banked master cell line using our automated platform to screen thousands of single cell clones. These permit transfection-free, large-scale production of lentiviral vectors for cell or gene therapies. This reduces the overall cost of goods and process complexity involved in clinical-scale viral vector production, while improving scalability and system reproducibility.
A further advantage of producer cell lines is the added potential for process development to improve viral titres once we've stably integrated your gene of interest, free from the process limitations imposed by transfection. Overall, lentiviral producer cell lines allow the consistent production of large quantities of lentiviral vector at lower cost.
OXGENE’s producer cell lines use our proprietary XLenti™ genetic system, which is optimised for translation efficiency, has Tet inducible VSV-G and Gag-Pol expression, and low homology between expression cassettes for an improved safety profile.
We use a fully defined, serum/animal component free process to generate both our packaging and producer GMP-compliant HEK293 cell lines. Cell lines do not contain the SV40 Large-T antigen and remain stable in the absence of antibiotic selection, minimising the regulatory risks in transitioning to clinical use.
View our most recent poster here.