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Transient AAV production

Industry leading technologies and services

Our industry leading technology platform provides flexible, high titre rAAV production, and is available for evaluation and licensing now. Our reconfigured Rep-Cap and modified Ad5 helper plasmids combined with our GMP banked clonal suspension HEK293 cell line offer gene therapy companies an improved AAV yield.

We offer expert cloning and engineering services to customise and optimise plasmids for expression of your gene of interest. Our process development services optimise for pre-clinical rAAV manufacture in our ISO:9001 accredited lab, and we manufacture up to 10L scale, before assisting technology transfer to your own facilities or CMO.

"OXGENE's AAV system is superior to off-the-shelf plasmid systems for AAV titres"

Andy Topping, Chief Scientific Officer at FUJIFILM Diosynth Biotechnologies

Transient AAV production

Our transient expression platform comprises optimised proprietary AAV plasmids and our GMP-banked clonal suspension HEK293 cell line. We have reconfigured the Rep and Cap genes to place Cap under the control of a strong CMV promoter, while Rep is expressed at a lower level via an EMCV internal ribosome entry site. We've also boosted expression of Assembly Activating Protein (AAP) from within the capsid ORF. This results in a higher viral titre, better per cell rAAV productivity and improved full:empty vector ratio. We've also improved adenoviral helper functionality by simplifying the Ad5 helper plasmid.

View our most recent rAAV vector system, AAV production, and HEK293 cell line posters.

From our experts to yours; we offer comprehensive technical support so you can have complete confidence in our technologies.

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Connex revolutionises DNA design, optimization and assembly by ensuring the highest levels of quality control, efficiency and project management available for any client.

OXGENE has fully integrated all its smart labs and robotic production processes within a single technology platform that allows real-time project updates for our customers.